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Properties of a Biocatalyst Based on Immobilized Recombinant Lipase Geobacillus stearothermophilus G3 in Synthesis of Methyl Esters of Fatty Acids
https://doi.org/10.18412/1816-0387-2015-6-90-96
Abstract
A heterogeneous biocatalyst BCL based on recombinant extracellular lipase of thermophilic bacterium Geobacillus stearothermophilus G3 with the activity of 23,6 act.un./g was prepared by covalent immobilization on aminated silica gel. The influence of the solvent nature, temperature (30–60 °C), proportion of water (1–10 %) and catalyst (0,25–25 %) on the yield of methyl esters of fatty acids (MEFA) was studied in the reaction of methanolysis of sunflower oil over the BCL catalyst. The observed maximal MEFA yield was 43 %. A high operation stability of the catalyst under study was demonstrated: The catalyst kept more than 50 % of its initial activity after 480 h operation (20 cycles) that makes it promising for synthesis of fatty acid esters used for production of biodiesel fuel.
About the Authors
Yu. V. Samoilova
Boreskov Institute of Catalysis, Novosibirsk
Russian Federation
Russian Federation
A. S. Piligaev
Boreskov Institute of Catalysis, Novosibirsk
Russian Federation
Russian Federation
K. N. Sorokina
Boreskov Institute of Catalysis, Novosibirsk; Institute of Cytology and Genetic, Novosibirsk; Novosibirsk National Research State University
Russian Federation
Russian Federation
A. S. Rozanov
Institute of Cytology and Genetic, Novosibirsk
Russian Federation
Russian Federation
S. E. Pel’tek
Institute of Cytology and Genetic, Novosibirsk
Russian Federation
Russian Federation
A. A. Novikov
Gubkin Russian State Oil and Gas University, Moscow
Russian Federation
Russian Federation
N. R. Almyasheva
Gubkin Russian State Oil and Gas University, Moscow
Russian Federation
Russian Federation
V. N. Parmon
Boreskov Institute of Catalysis, Novosibirsk; Novosibirsk National Research State University
Russian Federation
Russian Federation
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Review
For citations:
Samoilova Yu.V., Piligaev A.S., Sorokina K.N., Rozanov A.S., Pel’tek S.E., Novikov A.A., Almyasheva N.R., Parmon V.N. Properties of a Biocatalyst Based on Immobilized Recombinant Lipase Geobacillus stearothermophilus G3 in Synthesis of Methyl Esters of Fatty Acids. Kataliz v promyshlennosti. 2015;15(6):90-96. (In Russ.) https://doi.org/10.18412/1816-0387-2015-6-90-96
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