Comparison of Lactic Acid Production by Free and PVA-Cryogel-Immobilized Bacteria or Filamentous Fungi

There are results of investigations and comparative analysis of obtained process characteristics of lactic acid (LA) production from glucose within long-term batch process use of various samples of biocatalysts being bacterial L. сasei and filamentous fungus R. oryzae cells in concentrated (immobilized into poly(vinyl alcohol) cryogel or suspended) form. It was revealed for both bacteria and fungus cells that their use in immobilized into PVA-cryogel form is more reasonable as compared to concentrated suspended form since the period of their half-life-time increased in 6 and more times. Actually similar concentrations of LA were produced by immobilized fungus (920±5 g) and bacterial (895±5 g) biocatalysts during their 200 h-batch use, but twice increased level of substrate conversion into LA was obtained for fungus cells (0,92 g LA/g glucose), and the half-life-time of immobilized fungus biocatalyst appeared to be 10 times higher (80 days equal to 96 working cycles) then it was established for immobilized bacterial cells. The comparison between obtained and previously known from literature data showed that application of fungus cells immobilized into PVA-cryogel for LA-production is promising since the results of their usage surpass all processes by main characteristics: level of glucose conversion to LA and maximal accumulated product concentration.

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