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Improvement of the Efficiency of Bioconversion of Plant Materials under the Action of Mutant Strains of Cellulases Penicillium verruculosum
https://doi.org/10.18412/1816-0387-2016-5-83-88
Abstract
Plant biomasses are the predominant organic material on Earth. The efficiency of biocatalytic conversion of the plant materials is determined by the cost of their biotechnological processing to valuable commercial products (organic alcohols and acids, carbohydrates and hycrocarbons). The obtained recombinant strains Penicillium canescens produce, apart from their own enzymatic complex, heterological cellulases (mutant and non-mutant cellobiohydrolase I (CBHI) and endoglucanase II (EGII) P. verruculosum). Enzymatic agents (EA) prepared from recombinant strains P. canescens were more active to hydrolysis of crushed aspen wood. The yields of glucose and reducing sugars after 24–72 hour hydrolysis under the action of EA prepared from the recombinant strains were higher by на 48–52 % and 60–64 %, respectively, than under the action of EA prepared from the initial strainrecipient. Thus, introduction of site-specific mutations N45A and N194A for partial inhibition of the surface glycosilation led to a considerable increase in the yields of target CBHI and EGII.
About the Authors
A. S. Dotsenko
FRC Biotechnologies, Moscow; Moscow State University
A. M. Rozhkova
FRC Biotechnologies, Moscow
Russian Federation
Russian Federation
A. V. Gusakov
FRC Biotechnologies, Moscow; Moscow State University
Russian Federation
Russian Federation
A. P. Sinitsyn
FRC Biotechnologies, Moscow; Moscow State University
Russian Federation
Russian Federation
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Review
For citations:
Dotsenko A.S., Rozhkova A.M., Gusakov A.V., Sinitsyn A.P. Improvement of the Efficiency of Bioconversion of Plant Materials under the Action of Mutant Strains of Cellulases Penicillium verruculosum. Kataliz v promyshlennosti. 2016;16(5):83-88. (In Russ.) https://doi.org/10.18412/1816-0387-2016-5-83-88
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