Preparation of complex biocatalysts on the basis of enzyme specimens of recombinant fungal Penicillium verruculosum and their application in the hydrolysis of wood and agricultural industries waste

Currently, more and more interest grow to use a renewable biomass for producing fermentable sugars, organic and amino acids, which can be transformed into various useful products: polymers, materials for the pharmaceutical industry, food and fodder products. The conversion of vegetable polysaccharides to fermentable sugars is one of the most important stages in the processing chain of the bioconversion of cellulose wastes, taking place on the hydrolytic enzyme biocatalysts. Creation of recombinant strains P.verruculosum, producing cellulase complex with an additional increase in the activity of β-glucosidase, and xylanase is an urgent task in obtaining industrial strains of microorganisms that produce enzymes needed for high conversion of vegetable material. In this paper we obtain enzymes, biocatalysts for the conversion of biomass into simple sugars, with the increased activity of the heterologous exo- 1,4-β-glucosidase A.niger and endo-1,4-β-xylanase P.canescens using histone expression system in recombinant strains of P. Verruculosum; there is analysis of properties of new enzymes, studied fermentable ability of these drugs in the hydrolysis of shredded aspen and pine wood, which is waste of wood-processing, also a agricultural waste – bagasse. The utilization of these enzymes has the advantage compared to known analogs, because the compositions of new biocatalysts are more balanced, that offers you the highest yield of sugars without the cost of preparing the mixed individual drugs. It is shown that increased levels of xylanase and β-glucosidase (cellobiase) activities in the new biocatalyst resulting in 20–30 % increase yield of sugars and glucose reducing in the hydrolysis of powdered plant material – pine and aspen wood, also bagasse. The resulting enzyme preparations have potential applications in the woodworking industry and agriculture at the stage of polysaccharides cell wall making in fermentable sugars, such as the production of biobutanol. Appropriateness of using of new biocatalysts is a fermentable high performance of natural substrates, resulting from the balanced component of the enzyme preparations. Multienzyme complex of new biocatalysts can reduce the dosage of enzyme preparations in the hydrolysis process of vegetable waste.

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