Application of technology «fusion» to create a highly efficient biocatalysts based on recombinant strains of the fungus Penicillium verruculosum for the conversion of cellulose biomass

Hydrolysis of cellulose biomass under the action of biocatalysts (enzyme preparations) is one of the most advanced and environmentally friendly methods of obtaining a number of useful products. In this paper, a new approach is used to create a recombinant enzyme preparations with the desired properties by using fusion-constructs for gene cloning of target enzymes. A number of enzyme preparations is based on the strain of the fungus Penicillium verruculosum using fusionconstruct. The properties of these enzyme preparations are interest primarily as an additive to increase the capacity of the base hydrolytic cellulolytic complex P.verruculosum. Sharing new enzyme preparations and basic P.verruculosum allowed to increase its biocatalytic (hydrolytic) efficiency for the vegetable cellulose feed materials. By adding 20 % of the new enzyme preparation to the base without altering the total dosage of enzyme preparations in a reaction mixture the increasing of efficiency of hydrolysis of cellulose substrates (ground aspen wood and shredded pine wood deresined ) up to 70 %.

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